Project: Production of new bioactive compounds by plants and bacteria using new and improved halogenases

The in vitro use of halogenases is hampered by a fast inactivation of the enzymes under reaction conditions. To allow the use of halogenases in biotechnological processes, the reason for inactivation will be elucidated and stabel halogenase variants will be constructed. Since their application is also restricted by the high substrate specificity variants with a relaxed substrate specificity will be constructed. Already available tryptophan halogenases and variants from the project will be used to transform plants and bacteria producing tryptophan-containing secondary metabolites or indole derivatives to obtain new halogenated compounds produced in vivo. Using mild chemistries, the halogen atoms can be exchanged against different functional groups leading to novel compounds. Cultivation of transformed plants producing novel halogenated compounds will be optimised, the metabolic profile analysed and the biological and antioxidant activities of the compounds will be investigated.

Acronym NBCPBH
Website visit project website
Network ERA-IB-2
Call 4th ERA-IB Joint Call

Project partner

Number Name Role Country
University of Trás-os-Montes and Alto Douro Portugal
University of St. Andrews United Kingdom
John Innes Centre United Kingdom
Vita 34 Germany
Dresden University of Technology Coordinator Germany