Project: Surveillance Of mobiLome meDiated aNtibiotic rEsiStance Spread

Acronym SOLIDNESS (Reference Number: JPIAMR_2018_N013)
Duration 01/01/2019 - 31/10/2020
Project Topic Mobile genetic elements (MGEs) are DNA molecules that often carry important genes for the fitness of microorganisms, such as resistance and virulence genes, which may confer an adaptive advantage to recipient bacteria. Hence, they play a pivotal role in horizontal gene transfer. MGEs are one of the main players in antibiotic resistance dissemination and can be shared by different bacterial strains, species or even genera, which makes them one of the biggest concerns to healthcare stakeholders. MGEs are challenging to characterise through sequencing, due to their chimeric, modular and repetitive nature. Our main objective is to establish a network of excellence for surveillance of MGE-mediated antibiotic resistance spread. This network will improve the access to high-quality and curated MGEs sequencing data that will be shared at the international level. It will result in the production of documents (standard operating procedures, SOPs) by the network detailing 1) harmonisation of high-quality sequencing standards and protocols for MGEs detection; 2) definition of a bioinformatics workflow for MGEs sequence analysis from next-generation sequencing data; 3) definition of new sequence-based typing methods of plasmids for both Gram-positive and Gram-negative bacteria. Since the network includes a wide range of stakeholders with diverse expertise, including expertise in classical typing methods, “-omics”, bioinformatics and plasmid-detection, the combination of backgrounds will contribute to the creation of the high-quality and curated MGEs database from different sources. This proposal aims to track the evolution and spread of antimicrobial resistance and virulence in bacteria, mediated by MGEs, and in the future, find ways to prevent it.
Website visit project website
Network JPI AMR
Call 7th Joint Call of JPIAMR

Project partner

Number Name Role Country
1 University of Groningen Coordinator Netherlands
2 Freie Universität Berlin Observer Germany
3 University of Tübingen Observer Germany
4 University of Bern Observer Switzerland
5 University of Athens Observer Greece
6 Örebro University Hospital Observer Sweden
7 Federal University of Rio de Janeiro Observer Brazil
8 Universidade de Lisboa Observer Portugal
9 University of Helsinki Observer Finland
10 Istituto Superiore di Sanità Observer Italy
11 University of Bath Observer United Kingdom
12 Ben-Gurion University Observer Israel
13 Hvidovre Hospital Observer Denmark
14 University of Freiberg Observer Germany
15 Ramón y Cajal University Hospital Observer Spain
16 University of Ljubljana Observer Slovenia
17 Liverpool School of Tropical Medicine Observer United Kingdom
18 Universitätsklinikum Hamburg-Eppendorf Observer Germany
19 Sciensano Observer Belgium
20 University of Helsinki Observer Finland
21 National Institute for Public Health and the Environment Observer Netherlands