Project: Standardization of diagnostics and antimicrobial susceptibility testing and clinical interpretation in animal mycoplasmas.

Acronym	MyMIC (Reference Number: JPIAMR_NC_2022-019)
Project Topic	Animal mycoplasmas are major pathogens causing various diseases in livestock and pets, with significant economic losses for farmers. Their control relies largely on antimicrobial (ATB) use. Inappropriate and sustained use of ATB can select for resistance in the targeted bacteria, hence hampering treatment efficacy, but non-target microorganisms are also affected, including zoonotic/food-borne bacteria with significant impact on human health. The efficacy of ATBs in vivo can be indirectly assessed by in vitro susceptibility testing. However, existing standard procedures for AST could not be used for mycoplasmas, because of their different culture requirements and growth. The lack of standardized consensus procedures and quality control strains makes comparisons between studies difficult or impossible. Moreover, there are no clinical breakpoints or epidemiological cut-off (ECOFF) values determined specifically for animal mycoplasmas, which complicates the interpretation of results. The objective of this project is to set up a network of laboratories working on mycoplasma diagnostics and their susceptibility to ATBs to compare the different methods used and the results of minimum inhibitory concentrations (MIC). This project will lead to the drafting of guidelines for a standardized culturing, identification and determination of MICs. Moreover, the aggregation of MIC data obtained by the different laboratories will determine the first tentative ECOFFs for some Mycoplasma species to help clinical interpretation of MICs, and guide veterinarians toward a reasonable use of ATBs.
Website	visit project website
Network	JPIAMR-ACTION
Call	3rd JPIAMR-ACTION Joint Call 2022

Project partner

Number	Name	Role	Country
1	French Agency for Food, Environmental and Occupational Health and Safety	Coordinator	France
2	French Agency for Food, Environmental and Occupational Health and Safety	Observer	France
3	French Agency for Food, Environmental and Occupational Health and Safety	Observer	France
4	Royal GD	Observer	Netherlands
5	Royal GD	Observer	Netherlands
6	Clinic for Birds, Reptilies, Amphibians and Fish	Observer	Germany
7	Veterinary Medical Research Institute	Observer	Hungary
8	Universidad de Las Palmas de Gran Canaria	Observer	Spain
9	Universidad de Las Palmas de Gran Canaria	Observer	Spain
10	Universidad de Las Palmas de Gran Canaria	Observer	Spain
11	Ghent University	Observer	Belgium
12	Istituto Zooprofilattico Sperimentale delle Venezie	Observer	Italy
13	Istituto Zooprofilattico Sperimentale delle Venezie	Observer	Italy
14	Istituto Zooprofilattico Sperimentale delle Venezie	Observer	Italy
15	Animal and Plant Health Agency (Govt)	Observer	United Kingdom
16	Kimron veterinary Institute	Observer	Israel
17	Finnish Food Authority	Observer	Finland
18	Finnish Food Authority	Observer	Finland
19	University of Bern	Observer	Switzerland
20	University of Bern	Observer	Switzerland
21	National Veterinary Institute	Observer	Sweden
22	National Veterinary Institute	Observer	Sweden
23	National Veterinary Institute	Observer	Sweden
24	Asia-Pacific Centre for Animal Health	Observer	Australia
25	Asia-Pacific Centre for Animal Health	Observer	Australia
26	Asia-Pacific Centre for Animal Health	Observer	Australia
27	Asia-Pacific Centre for Animal Health	Observer	Australia
28	Asia-Pacific Centre for Animal Health	Observer	Australia
29	National Veterinary Research Institute Pulawy	Observer	Poland
30	Asia-Pacific Centre for Animal Health	Observer	Australia
31	Asia-Pacific Centre for Animal Health	Observer	Australia
32	Asia-Pacific Centre for Animal Health	Observer	Australia
33	Asia-Pacific Centre for Animal Health	Observer	Australia
34	National Veterinary Research Institute	Observer	Poland
35	University of Maiduguri	Observer	Niger
36	University of Liverpool	Observer	United Kingdom
37	Veterinary Medical Research Institute	Observer	Hungary
38	Veterinary Medical Research Institute	Observer	Hungary
39	Veterinary Medical Research Institute	Observer	Hungary
40	VetAgro Sup	Observer	France
41	University of Agriculture Peshawar	Observer	Pakistan
42	University of Agriculture Peshawar	Observer	Pakistan
43	University of Veterinary Medicine, Vienna	Observer	Austria
44	Centro National de Sanidad Agropecuaria	Observer	Cuba
45	University of Ghent	Observer	Belgium
46	Centre de Coopération International en Recherche Agronomique pour le Développement	Observer	France
47	Centre de Coopération International en Recherche Agronomique pour le Développement	Observer	France
48	Centre de Coopération International en Recherche Agronomique pour le Développement	Observer	France