Project: High-Throughput, High-Content Screening of human neuroectodermal organoids for innovative drug discovery in neurodegenerative diseases

Acronym 3DMinBrain (Reference Number: 99)
Duration 01/10/2015 - 30/09/2019
Project Topic 3D MiniBrain is an ambitious innovative and multidisciplinary collaborative project designed to provide the proof of concept of an original predictive experimental model based on human minibrains (derived from induced pluripotent stem cells, iPSCs), the natural complexity of which will be addressed by automated 3D imaging, to study neurodegenerative disorders. Its main goal is to establish and standardize relevant complex cerebral organoid models to decipher the roles of the main genetic risk factors for Alzheimer’s disease (AD) and Parkinson’s disease (PD) according to original hypotheses developed by partners (post-GWAS). Accordingly, this application fits with topic 1 and topic 3 of this call and will allow to: - Specifically characterize a pathway at the interplay between the Amyloid-ß (Aß) and Tau pathology in AD; - Specifically characterize the pathways that lead to α-synuclein aggregation and toxicity in late onset PD; - Assess the aggregation and seeding hypothesis of AD and PD and to test the limits of ability to be infected by human prion strains; - Use models developed in the previous objectives to develop high throughput screening. Workpackages are proposed 1) to study the model in vitro, 2) to identify targets/genes, 3) for seeding experiments and 4) compound screening. The teams involved are known for their excellence in the academic field and are also highly experienced in the transfer of knowledge and technology to the industry to which will be proposed the best models as a service at the end of the project.
Network JPco-fuND
Call Neurodegenerative diseases: risk and protective factors, longitudinal cohort approaches and advanced experimental models

Project partner

Number Name Role Country
1 Commissariat à l'energie Atomique Coordinator France
2 Deutsches Zentrum für Neurodegenerative Erkrankungen Partner Germany
3 Institut Pasteur de Lille Partner France
4 Scuola Internazionale Superiore di Studi Avanzati-SISSA Partner Italy