Project: Antibiotic REsistaNce and Pathogenic Signature in Marine and Freshwater Aquaculture Systems

Acronym ARENA (Reference Number: ID 329)
Duration 01/09/2021 - 31/08/2024
Project Topic The project aims to improve the quality assessment of aquaculture practices and products by exploring the fate of antibiotic and microbial contaminants across the water cycle. Contamination levels will be assessed from sources (effluents, river outflows) to end points (aquaculture plants) and final food products (e.g., fish fillets), along with the antibiotic resistome and pathogenic signature in farm surrounding aquatic environments in open (i.e., mariculture) and recirculating aquaculture systems (i.e., RAS). For mariculture only, the assessment of the same contaminants will be also performed in benthic biota beneath fish cages; moreover, in order to take into account possible seasonal variations, we will perform analyses at contrasting seasons (e.g., summer, generally characterized by a higher anthropogenic pressure, and winter, with a lower anthropogenic pressure). Novel early-warning tools for the rapid detection of antibiotic residues (flow cytometry-based), antibiotic-resistance genes (high-throughput sequencing-based), and microbial pathogens (sensor-based) in environmental and biological samples will be optimized. ARENA will contribute to elucidate the significance of antibiotic-related issues, finally providing a cross-disciplinary approach for risk assessment and future operative efforts to mitigate the magnification of antibiotic resistance and pathogenicity in aquaculture settings.
Project Results
(after finalisation)
We expect to provide knowledge and tools to rapidly detect AMR, microbial and AB contamination in aquaculture. Our results will be key for improving the whole aquaculture value chain by ensuring an overall safer food production, a higher environmental protection and an effective guide for policymakers in treatment strategies, epidemiological surveillance and resistance evolution. The project aims at bringing about 2 different embodiments of a sensing device for bacteria (for stationary monitoring and for on-site sampling), together with a protocol for the up-scalable synthesis of bacterial-receptor chips
Network AquaticPollutants
Call 1st AquaticPollutants Joint Call 2020

Project partner

Number Name Role Country
1 National Research Council - CNR Coordinator Italy
2 Catalan Institute for Water Research (ICRA) Partner Spain
3 University of Applied Sciences Aachen Partner Germany
4 University of Liège Partner Belgium
5 Katholieke Universiteit Leuven (KU Leuven) Partner Belgium